by TER ASSA — interest into an expression vector that is then transferred into ... Luciferase reporter technology gives the researcher unparalleled sensitivity, dynamic range, versatility and ease of ... stable cell line with a CRE/promoter-luciferase introduced.. Results 1 - 12 of 42 — ... activation of intracellular signal transduction pathways. Cotransfection of a cis-​reporter plasmid with an expression plasmid shows direct or .... The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency.
cre luciferase reporter plasmid
468 products — ... next to the gene promoter of interest within a vector and transfected to a cell line. ... CRE Luciferase Reporter-HEK293 Cell Line ... The CRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses.. Cyclic AMP Response Element (CRE/CREB) CRE-Luc, CREB-Luc Response Element ... A luciferase reporter gene can be engineered into organisms or cells, allowing ... Vector Type: pLT-Luc or pLT-Luc-Marker (see the vector map above).. Results 1 - 12 of 42 — ... activation of intracellular signal transduction pathways. Cotransfection of a cis-​reporter plasmid with an expression plasmid shows direct or .... by Y Chen · Cited by 10 — Both cell lines were observed a cAMP-inducible luciferase expression after transfection of the reporter plasmid CRE-luc. Stimulated by 5-.. Luciferase reporter assay systems are successfully used in the drug discovery process against ... in the same vector as CRE-firefly luciferase. However, no such​.. by LR Watkins · 2021 · Cited by 1 — For CRE and NFAT luciferase reporter assays, cells were incubated ... Plasmids encoding the following GPCRs were obtained from cDNA .... by SJ Kaczmarczyk · 2003 · Cited by 15 — The use of cre recombinase to activate or inactivate gene expression ... Transfections with luciferase reporter plasmids were performed 2 h later .... Sep 3, 2010 — Binding of transcription factors, such as the cAMP response element binding protein (CREB), to the CRE sequence allows Metridia luciferase ( .... The pCRE Tluc16-DD Vector is a transcriptional reporter vector designed to monitor the activation of cAMP-binding protein (CREB) and cAMP-mediated signal .... by Z Cheng · 2010 · Cited by 133 — Keywords: Luciferase, Reporter assays, GPCR, profile of receptor/G protein coupling. INTRODUCTION ... cluding cAMP response element (CRE), nuclear factor of ... reporter vector and receptor (D1 dopamine receptor and m3.. 2.1 Promoter Studies with Dual-Luciferase Reporter Assays. 12. 2.2 Signal ... 2.3 Vector map of a co-reporter plasmid for dual-reporter assays. In this vector, the reporter ... downstream genes. CRE: Cyclic-AMP response element; SRE: Serum.. Luciferase Reporters in Circadian Research · GFP tagging (Green Fluorescent Protein fusion) · What is .... Cignal Reporter Assays are based on dual-luciferase technology, and generate exceptionally ... control, C a constitutively expressing GFP construct containing Monster Green® ... elevate intracellular cAMP, compared to the CRE reporter that​.. by Q Shan · 2010 · Cited by 11 — Luciferase assay. Cells were transfected with the CRE-luciferase reporters and EF1-LacZ plasmids. The EF1-LacZ plasmid contains the β- .... by S Li · 2018 · Cited by 14 — In the conditional Cre/loxp reporter system, the first reporter gene is flanked by two loxp ... The GFP from the jellyfish, Aequorea victoria, discovered in 1962 by ... Szyska et al69 generated dual-luciferase reporter mouse model .... The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The​ .... by H Li · 2020 — (F) Construction of PA-Dre in a single plasmid. Schematic ... fold) but lower luciferase reporter activity than Magnets–PA-Cre. (SI Appendix, Fig.. Componets 1 CRE(1)Luciferase Reporter Vector (20ul of 500 ng/ul) ... cAMP response element (CRE), including Jun N-terminal kinase (JNK), p38, and.. by D Akhmedov · 2016 · Cited by 7 — The ROSA26-CRE-luc mouse line is a useful resource to study ... we obtained a validated CREB-activated luciferase reporter plasmid from .... The GLuc-ON™ CREB TRE lentiviral clone contains tandem repeats of CRE upstream of ... LF061, Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns), Detects ... HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin .... by I Manual — Plasmid Information). In addition to the luciferase reporter format, the CRE, AP-1, NF-kB and. NFAT cis-reporting plasmids are available with an hrGFP reporter.. ... transduction and expression of the target genes as well as antibiotics resistant genes or reporters. ... Cre (PGK) Firefly Luciferase (CMV) Lentivirus (2x25uL).. Products 1 - 25 of 44 — The CRE motif (as Transcription response element, TRE) provides a system for study ... report (Luciferase, Renilla Luc) or a fluorescent report (GFP, RFP) or a ... The luciferase signal can be measured via Luciferase assay.. Jun 28, 2021 — The novel cyclic adenosine monophosphate (cAMP)-responsive luciferase reporter exhibits optimal performance in high-throughput screens of .... The pGL4.29[luc2P/CRE/Hygro] Vector contains a cAMP response element (CRE​) that drives the transcription of the luciferase reporter gene luc2P (Photinus .... by I Manual — Plasmid Information). In addition to the luciferase reporter format, the CRE, AP-1, NF-kB and. NFAT cis-reporting plasmids are available with an hrGFP reporter.. CREB Responsive Luciferase Reporter HEK293 Stable Cell Line is derived from ... Cyclic AMP response element (CRE)-binding protein (CREB) is a ... line by co-​transfecting CREB luciferase reporter vector and hygromycin expression vector.. This strain serves as a Cre reporter strain. When used ... strain, successful Cre-​mediated excision is indicated by luciferase expression in Cre-expressing tissues​.. Article Snippet: Expression of a CRE-luciferase reporter gene was induced by cotransfection with the pFC-PKA plasmid expressing the catalytic subunit of .... by M Safran · 2003 · Cited by 132 — using a viral vector or by mating to one of an expanding number of transgenic Cre mouse strains. Bioluminescent imaging based on luciferase expression is a .... by M Schwaninger · 1993 · Cited by 89 — Fax: 551-399652. The abbreviations used are: CRE, cAMP response element; CaM ... Plasmid Construction-The plasmids -350GluLuc and. 5xCRET81Luc ... acetyltransferase assay (20) and the luciferase assay were performed as previously .... Browse Addgene's collection luciferase plasmids assays transcriptional regulation. ... 106460, pCR3.1-CREM2 luc, luciferase reporter, CMV promoter (​CRE site .... by MD Conkright · 2003 · Cited by 650 — late CRE-dependent transcription via a phosphoryla- tion-independent ... taining EVX-1 luciferase reporter vector (Conkright et which CREB .... Luciferase reporter assays are used widely to investigate cellular signaling ... expression in mammalian cells, and the pGL4 plasmid backbone is engineered.. by OG CHEPURNY · 2007 · Cited by 58 — Plasmid DNA for all experiments was obtained from transformed ... Characterization of a novel luciferase reporter incorporating the CRE of .... The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency.. The luciferase gene is subcloned from a pGL3-basic vector and inserted into a p4xCRE-blax vector and ... Luciferase Assay of CHO-K1/CRE/Luc Reporter Cells:​.. by TER ASSA — interest into an expression vector that is then transferred into ... Luciferase reporter technology gives the researcher unparalleled sensitivity, dynamic range, versatility and ease of ... stable cell line with a CRE/promoter-luciferase introduced.. ... Plasmid or Mock Plasmid (A) Hek293T, CHO or Huh-7 cells were transfected with CRE-luciferase reporter constructs and a human TGR5 expression plasmid .... Dec 18, 2020 — COS-2 cells were transfected with plasmids encoding either the firefly luciferase or GFP reporter of Cre recombinase activity described above.. The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency.. by Y Chen · 2015 · Cited by 10 — Both cell lines were observed a cAMP-inducible luciferase expression after transfection of the reporter plasmid CRE-luc. Stimulated by 5-HT for .... by M Ohtsuka · Cited by 4 — Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused ... Luc assay was performed using a kit (Dual-Luciferase Reporter Assay ... 4f4a45da30 17
luciferase reporter plasmid, luciferase reporter plasmid transfection, luciferase reporter plasmid addgene, nfkb luciferase reporter plasmid, dual luciferase reporter plasmid, nfat luciferase reporter plasmid, cre luciferase reporter plasmid, ap 1 luciferase reporter plasmid, pgl3 luciferase reporter plasmid, promega luciferase reporter plasmid, luciferase reporter vector, luciferase reporter vector cloning, luciferase reporter vector promoter